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Enabling PCR robustness: 100% contamination-free PCR reagents

Conventional PCR reagents contain significant contaminations of DNA from the production strain, E. coli. In addition, some dNTP manufacturers use enzymatic nucleotide modification processes and even molecular-grade water can contain considerable amounts of residual microbial DNA. Based on a proprietary enzyme production and purification technologies Molzym has developed a 100% DNA-free Taq-polymerase. In addition, Molzym provides the complete range of 100% DNA-free PCR reagents (water, buffers, BSA, dNTPs) and a service for custom-based mastermix development and purification.


View tutorial on contamination-free PCR reagents:



Selected applications:

  • Broad-range PCR applications
  • 16S/18S rDNA PCR and Real-Time PCR applications
  • Various assays in molecular microbiology
  • Quality control in pharma and biotechnology
  • Next-generation sequencing
  • Whole genome amplification

Selected Publications:

  1. Mühl H, Kochem AJ, Disqué C, Sakka SG (2010) Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood. Diag. Microbiol. Infect. Dis. 66, 41-49.

  2. Jones HE, Harris KA, Azizia M, Bank L, Carpenter B, Hartley JC, Klein N, Peebles D (2009) Differing prevalence and diversity of bacterial species in fetal membranes from very preterm and term labor. PLoS One 4, e8205; doi:10.1371/journal.pone.0008205.

  3. Handschur M, Karlic H, Hertel C, Pfeilstöcker M, Haslberger AG (2008) Preanalytic removal of human DNA eliminates false signals in general 16S rDNA PCR monitoring of bacterial pathogens in blood. CIMID 32, 207-219.


Licensing inquiries: Jan Detmers, Ph.D., This e-mail address is being protected from spambots. You need JavaScript enabled to view it