NGS - Ultra-Deep Microbiome Sequencing

 

Ultra-Deep Microbiome Sequencing:

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  • Unlocking the power of NGS for microbiome research
  • Enabling ultra-deep analysis of microbiomes
  • Studying disease-associated and polymicrobial infections
  • Validated for a wide range of clinical specimens

Deep Microbiome Sequencing

ngs_workflow_fig1 Next generation sequencing platforms (NGS) are currently revolutionizing the way researchers can study microbial colonization, infections and microbial communities (microbiomes). In microbiome research NGS has become a valuable tool for studying the microbial community [1], [2], [3]. Deep sequencing approaches have revealed new species and have given new insight into polymicrobial infections. Overall, deep sequencing of microbiomes has proven a huge potential for improving treatments (among others) against cystic fibrosis, gastric ulcer or vaginal infections. Over the past 25 years Sanger-based sequencing has been the “gold standard” and has been used for the characterization of microbiomes. Unfortunately Sanger sequencing is limited in terms of sequencing depth and sequence information is often limited to the most predominant species. In contrast NGS-based deep sequencing allows for in depth analysis of microbiomes on a genetic level. Nevertheless the depth of any microbiome deep sequencing approach is limited by factors like human DNA, reagents contaminations, and lack of calibrators. (Figure 1 summarizes the steps that can dramatically influence the quality of deep sequencing results, including sample processing, nucleic acid extraction and amplification). Therefore, Molzym has launched a range of innovative tools and services enabling the study of microbiomes.

 

Ultra-Deep Microbiome Sequencing - Unlocking the power of NGS

NGS-based microbiome bioanalysis aims to characterize the structure and dynamics of the living microbiome. DNA from dead microorganisms and human sources can significantly disturb the picture gained by deep sequencing results and significantly lowers the analytical sensitivity. Molzym offers research tools addressing current limitations in deep microbiome sequencing with respect to the removal of DNA from human cells and dead microbial cells, universal lysis of Gram-negative and Gram-positive bacteria and fungi and supply of contamination-free extraction and PCR reagents.

 

Ultra-Deep Microbiome Prep

The Ultra-Deep Microbiome Prep is the ultimate solution combining protocols for removal of host DNA and "dead" DNA and extraction of enriched microbial DNA for microbiome analysis including DNA-free buffers and plastics. The Ultra-Deep Microbiome Prep allows for significantly deeper sequencing compared to conventional DNA isolation. 

>> Ultra-Deep Microbiome Prep Kit

 

Contamination-free PCR Reagents

Molzym’s purification technology removes traces of contaminating DNA from PCR reagents and supplies highly active enzymes and assays for research applications. This results in enhanced reliability in amplification of sequences originating from the community of bacteria and fungi.

>> Contamination-free PCR reagents

 

Whitepaper: New Developments in DNA Extraction For Microbiome Analysis

This whitepaper gives an overview of new solutions for enrichment and extraction of microbial DNA, removal of irrelevant non-target host DNA and ways to discriminate for DNA from live microbes.

 >>Download PDF

 

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References

[1] The Human Microbiome Project: http://commonfund.nih.gov/hmp/index.aspx

[2] Siqueira JF et al. (2012) J. Oral Microbiol. 4:10743

[3] Rogers GB, Bruce KD (2010) Mol. Diagn. Ther. 14:343-350

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Unlocking the power of NGS for microbiome research

Enabling ultra-deep analysis of microbiomes

Studying disease-associated infections and polymicrobial infections

Validated for a wide range of clinical specimens