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MolYsis – Targeted Pathogen DNA Isolation

Kit
Specimen
volume (ml)
Combinable with other DNA isolation kits
 
MolYsis Basic
pediatric blood
0.2
+
MolYsis Basic5
adult blood
5
+
MolYsis Complete5
adult blood
1 or 5
complete system
MolYsis Plus
Blood culture
0.2
complete system

MolYsis is the new technology for enhanced PCR analysis of pathogens in blood and other specimens. By the simple treatment of blood cell lysis and human DNA degradation, pure pathogen DNA is isolated. The result is an increase of the PCR analytical sensitivity of up to 20,000-fold compared to total DNA isolation systems, allowing the detection of 1 genome equivalent per assay. No false signals arise as the result of unspecific primer binding to human DNA.

MolYsis Basic kits are combinable with any DNA isolation system (e.g. Qiagen DNeasy/QiaAmp, Roche High Pure PCR Template Preparation Kit, Epicentre Masterpure DNA Purification Kit). Also automated DNA isolation systems can be switched (e.g. Roche Magnapure, FujiFilm QuickGene-810, Chemagen Chemagic MSM I, PSS Magtration, Biomerieux easyMag). MolYsis Complete5 is a kit that includes all steps from human DNA removal to the isolation of pathogen DNA from 1 ml or 5 ml whole blood. The MolYsis Plus kit is adapted to blood cultures, removing strong PCR inhibitors.
    Features of MolYsis
    - Infection PCR diagnosis
    - Human DNA removal
    - Pathogen enrichment
    - PCR inhibitors removal
    - Universal pathogen lysis
    - Pathogen DNA within 2 h
    - 0.2 to 5 ml sample
    - Combinable with any DNA isolation kit, including automated systems
    Specimens
    - Whole blood
    - Cerebrospinal fluid
    - Knee aspirates