DNA-free PCR Reagents
MolTaq 16S and Mastermix 16S
New: Short tutorial on contamination-free PCR-mastermixes.	Benefits:
	DNA-free Highly active Ultra-sensitive bacteria detection Suitable for Real-time PCR

There is a strong risk of false positive signals as a cause of unspecific amplification from contaminating DNA in PCR reagents, including the polymerising enzyme, primers and dNTPs.

Molzym’s purification technology removes traces of DNA from the PCR reagents and supplies a highly active enzyme in the products. This results in enhanced sensitivity and reliability of bacteria detection in the assays.

Solutions are offered to meet your requirements ranging from DNA-free Taq polymerase, MolTaq 16S and CE-IVD certified MolTaq IVD to mastermixes in its various designs, Mastermix 16S kits. Assays are available, Mastermix 16S Primer and Mastermix 16S Complete, for the detection of bacteria by the amplification of a highly variable region of the 16S rRNA gene.

If you want to use your own primers, we offer Mastermix 16S Basic and Mastermix 16S Dye kits which contain all components for the PCR reaction except primers.

Sequencing of amplicons combined with online homology BLAST search is an option for the identification of bacteria detected by Molzym assays, Mastermix16S Complete or Mastermix16S Primer. Molzym offers sequencing primers that allow the sequence analysis and identification of bacteria. The primers bind to conserved regions within the amplified DNA (approx. 460 bp) enabling sequencing of highly discriminative sequences for strain identification. Sequencing primer SeqGP16 covers a variety of Gram-positive organisms, including staphylococci, streptococci and enterococci. Sequencing primer SeqGN16 on the other hand allows the identification of many pathogenic Gram-negative bacteria, including enterobacteria and pseudomonads. For more detailed list of strains see the FAQ.

Sequences generated by using the sequencing primers can be easily analysed by referring to our free sequence alignment software, SepsiTest-BLAST. If you like to know more about this software, please click here.