Kits - DNA-free PCR Reagents
A series of kits are especially dedicated to the sensitive and specific detection of bacterial pathogens in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance. MolTaq 16S is the Taq DNA polymerase of choice if you experience contamination problems with other standard Taqs. Mastermixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, MgSO4 (3 mM final) and BSA. Mastermix 16S Basic and Mastermix 16S Dye are available for assays using custom primers, and Mastermix 16S Primer and Mastermix 16S Complete for universal detection of bacteria in samples by PCR and Real-Time PCR, respectively. The mastermixes are 2.5x concentrated.
Product Overview:
| Product | Application | Shopping Cart |
| MolTaq 16S |
bacteria and fungi detection | order |
| Mastermix 16S Basic | custom PCR assays | order |
| Mastermix 16S Dye | custom Real-Time PCR assays | order |
| Mastermix 16S Primer |
universal bacteria PCR assay | order |
| Mastermix 16S Complete | universal bacteria Real-Time PCR assay | order |
| Component | MolTaq 16S MolTaqIVD |
Mastermix 16S Basic |
Mastermix 16S Dye |
Mastermix 16S Primer |
Mastermix 16S Complete |
| Taq DNA polymerase, buffer, PCR-grade water | + | + | + | + | + |
| dNTPs | - | + | + | + | + |
| fluorescent dye | - | - | + | - | + |
| 16S rRNA gene primers | - | - | - | + | + |
DNA-free water is high quality PCR-grade water that is used in the setup of PCR and Real-Time PCR assays demanding high accuracy of analysis. Applications comprise quality control of manufacturing processes in pharma and biotechnology, contamination control of food products, environmental analysis, molecular infection diagnosis, SNP and other hereditary mutation analyses, and research. DNA-free water allows the precise molecular analysis with utmost reliability. This product is manufactured under strict quality control management guaranteeing the absence of human and microbial DNA contamination (<3% false-positive rate).
| Product | Content | Shopping Card |
| DNA-free water, PCR-grade | DNA-free water, 10x 1.7ml | order |
Sequencing Primers (in conjunction with universal PCR assays, Mastermix 16S Primer and Mastermix 16S Complete):
| Product | Content | Application | Shopping Cart |
| Set of sequencing primers | SeqGP16 and SeqGN16 (10 pmol/µl) |
sequencing of amplicons |
order |

