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Kits - DNA-free PCR Reagents

A series of kits are especially dedicated to the sensitive and specific detection of bacterial pathogens in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance. MolTaq 16S is the Taq DNA polymerase of choice if you experience contamination problems with other standard Taqs. Mastermixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, MgSO4 (3 mM final) and BSA. Mastermix 16S Basic and Mastermix 16S Dye are available for assays using custom primers, and Mastermix 16S Primer and Mastermix 16S Complete for universal detection of bacteria in samples by PCR and Real-Time PCR, respectively. The mastermixes are 2.5x concentrated.

 

Product Overview:

Product Application Shopping
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MolTaq 16S
bacteria and fungi detection order
Mastermix 16S Basic custom PCR assays order
Mastermix 16S Dye custom Real-Time PCR assays order
Mastermix 16S Primer
universal bacteria PCR assay order
Mastermix 16S Complete universal bacteria Real-Time PCR assay order

 



Component MolTaq 16S
MolTaqIVD
Mastermix 16S
Basic
Mastermix 16S
Dye
Mastermix 16S
Primer
Mastermix 16S
Complete
Taq DNA polymerase, buffer, PCR-grade water + + + + +
dNTPs - + + + +
fluorescent dye - - + - +
16S rRNA gene primers - - - + +

 


DNA-free water is high quality PCR-grade water that is used in the setup of PCR and Real-Time PCR assays demanding high accuracy of analysis. Applications comprise quality control of manufacturing processes in pharma and biotechnology, contamination control of food products, environmental analysis, molecular infection diagnosis, SNP and other hereditary mutation analyses, and research. DNA-free water allows the precise molecular analysis with utmost reliability. This product is manufactured under strict quality control management guaranteeing the absence of human and microbial DNA contamination (<3% false-positive rate).

Product Content Shopping Card
DNA-free water, PCR-grade DNA-free water, 10x 1.7ml order

 


Sequencing Primers (in conjunction with universal PCR assays, Mastermix 16S Primer and Mastermix 16S Complete):

Product Content Application Shopping
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Set of sequencing primers SeqGP16 and SeqGN16 (10 pmol/µl)
sequencing of amplicons
order