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Molecular Microbiology News Vol.4/2018 |
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Hot Topic: New Solutions for 16S Metagenomic Sequencing |
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Publication |
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Depletion of Human DNA and Reduction of Bacterial Contamination towards a Standardised Method for Infection Diagnosis with Next Generation Sequencing (Edelmann et al. 2018) Edelmann and colleagues compared 4 DNA extraction kits and the influence of human DNA removal for use in low microbial load microbiome analysis. Additionally, two master mixes were tested for bacterial DNA contamination. The authors found out that DNA extraction and master mix reagents have a huge impact on the contamination signature in sequencing reads. They resumed that MolYsis-SelectNA™plus together with NGS Mastermix 16S V3/V4 aid an efficient workflow for NGS towards a standardised method in microbiome infection diagnosis. An efficient workflow towards a standardised use of NGS in infection diagnosis was proposed. [Note: NGS Mastermix 16S V3/V4 was renamed in NGSeq 16S V3/V4] |
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New Product |
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NGSeq 16S V3/V4 Amplicon PCR for 16S library preparation The ultra-clean NGSeq 16S V3/V4 is a master mix for the amplification of eubacterial DNA with primers specific for the V3/V4 region of the 16S rRNA gene. Primers also include overhang adaptor sequences suitable for the library preparation for 16S metagenomic sequencing with Illumina® MiSeq system. The NGSeq 16S V3/V4 includes everything necessary to quickly perform the Amplicon PCR as first stage of the 16S library preparation protocol. Product features: · Microbial DNA-free · Highly active hot start Taq polymerase · Up to 40 cycles ultra-sensitive amplification of bacterial DNA · For Illumina® MiSeq system |
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Related Products |
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MolYsis-SelectNA™plus Automated microbial DNA enrichment and isolation from fluid and tissue specimens, removal of non-target host DNA Molzym’s MolYsis-SelectNA™plus is a kit for the contamination-free automated extraction of bacterial and fungal DNA allowing significantly deeper sequencing compared with conventional DNA isolation systems. Product features: · Extraction on SelectNA™plus benchtop robot · Isolation of enriched microbial DNA · Removal of non-target human/animal DNA (MolYsis™ technology) · Flexibel: 1-12 samples per run · Contamination-free DNA isolation reagents, buffers and columns |
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DNA-free Water Ultra-pure, DNA-free water for precise molecular analysis Molecular analysis can be prone to false-positive results due to reagent-based contaminations with microbial DNA. DNA-free water is ideally suited to be used in all kind of molecular microbiology applications. Product features: · Sterile, PCR-grade water · Guaranteed microbial DNA-free · PCR and Real-Time PCR tested (≥40 cycles) · High and consistent product quality |
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New: Follow Molzym’s Blog! Stay informed on topics covering pathogen enrichment and isolation from clinical samples, automation, molecular diagnostics and Next-Generation Sequencing. Get practical tips and insights into our solutions. |
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Dear reader, Welcome to this edition of Molecular Microbiology News. With this newsletter we want to keep you updated on the latest developments in molecular detection of microbial infections. Best regards, Michael Lorenz, Ph. D. (Editor in Chief) |
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This information is brought to you by Molzym GmbH & Co. KG, Mary-Astell-Str.10, D-28359 Bremen, Germany // If this information is not of interest to you, please disregard and we apologize for any inconvenience this may have caused. If you want to unsubscribe from this newsletter please click unsubscribe. |
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