NGSeq 16S V3/V4
Amplicon PCR for 16S library preparation - For Illumina® MiSeq system
Characters and benefits:
The NGSeq 16S V3/V4 is an ultra-clean master mix for the amplification of eubacterial DNA with universal primers targeting the V3/V4 region of the 16S rRNA gene. The primers include the adaptor sequences suitable for the library preparation for 16S metagenomic sequencing with the Illumina® MiSeq system. The sequences are based on “16S Metagenomic Sequencing Library Preparation”  and combinable with the indices for sequencing on the Illumina® MiSeq system.
Fig.1: Schematic drawing of 16S Amplicon PCR using NGSeq 16S V3/V4
The NGSeq 16S V3/V4 contains everything necessary to quickly perform the Amplicon PCR and thus the first stage of the 16S library preparation . The microbial DNA-free reagents offer the possibility to run up to 40 cycles for a higher and more sensitive amplification. Sequencing of contaminations from reagents is prevented allowing a representative view into the bacterial community. Optional real-time PCR can be used to check samples prior to sequencing.
Molzym offers a wide range of DNA-free PCR reagents and assays for sensitive and broad-range PCR applications. Click here for more information.
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|NGSeq 16S V3/V4||
Master mix for preparation of 16S rRNA gene amplicons for Illumina® MiSeq system
 16S Metagenomic Sequencing Library Preparation; Part # 15044223 Rev.B; https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf