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 The PCR-based analysis of low load bacterial and fungal DNA demands a Taq DNA polymerase that is highly active over extended cycle numbers, and at the same time free of contaminating microbial DNA.
In this application note, Linow [1] provides an overview of the specifications of Molzym’s newly developed Hot MolTaq 16S/18S. Discussed characters cover the amplification activity over 40 cycles against different target amounts, suppression of non-specific amplifications and the absence of microbial contaminations in negative controls. Further, a comparative study of Molzym’s Hot MolTaq 16S/18S with other hot start Taq DNA polymerases declared as low contaminated is shown.
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