SepsiTest™-UMD: Unbiased rDNA PCR Pathogen Diagnosis |
05 November 2018 |
Molzym has developed the SepsiTest™-UMD CE IVD system that uses broad-range 16S and 18S rRNA gene PCR and sequencing analysis of eubacteria and fungi, respectively. In this context the removal of host DNA and thereby enrichment of microbial DNA plays an important role for the enhancement of analytical sensitivity (see blog post “MolYsis™ Host DNA Removal – Effect on Pathogen Analysis”). After DNA extraction and PCR (4 hours) a first result on the presence or absence of eubacteria and fungi is obtained. Validation of the results is achieved by supplied extraction and PCR run controls (positive, negative). Amplicons from positive assays are Sanger sequenced, and a BLAST analysis by Molzym’s online tool, SepsiTest™-BLAST, identifies the species on the grounds of a quality-edited library of >7,000 entries of 16S and 18S rRNA genes (see Fig. 1). |
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Fig.1: Overview of the unbiased diagnostic approach of SepsiTest™-UMD. |
The broad-range identification character of SepsiTest™-UMD has been demonstrated in evaluations including clinical fluid and tissue specimens as well as other materials. The identification of so far 1,359 microorganisms from 303 bacterial and 40 fungal genera emphasizes the unbiased concept of SepsiTest™-UMD diagnosis (inquire the Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein!). For further information on clincial evaluations of SepsiTest™-UMD follow the blog “Molecular Diagnostics”. For more detailed information about SepsiTest™-UMD CE IVD please refer to our application note or visit the product site. |
Application Note Brochure Product Information |