Thank You for Visiting US at ECCMID 2018 |
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15 May 2018 | |
We were delighted to present our latest developments for unique automated culture-independent pathogen DNA extraction and broad-range molecular diagnosis of bacteria and fungi to this international audience. We want to thank everyone who visited us at our booth #159 and hope that you enjoyed the meeting as much as we did! |
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In the oral session “Innovations in molecular pathogen detection”, Dr. Rubalskii [1] from Hannover Medical School (Germany) presented his results on a recent study on infectious endocarditis (IE). Automated pathogen enrichment and DNA extraction on the SelectNA™plus robot was used in combination with broad-range 16S/18S rDNA PCR and sequencing analysis (Micro-Dx™) to diagnose 97 samples from 52 patients under suspect of IE. Specimens included mainly excised heart valves and blood, but also pacemaker electrodes, haematomas, pericardial abscesses and thrombi. The SelectNA™plus/Micro-Dx™ analysis resulted in 58% positivity as compared to 42% by culture. The good clinical performance makes SelectNA™plus/Micro-Dx™ a promising tool for culture-independent molecular diagnosis of IE infections as concluded by the speaker. |
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References | |
[1] Rubalskii E, Ruemke S, Salmoukas C, Baussmerth C, Keim S, Disqué C, Haverich A, Kühn C (2018) Broad-Range 16S/18S PCR and Sequencing Diagnosis of Infectious Endocarditis Using Automated Pathogen Enrichment and DNA Extraction. Oral presentation ECCMID 2018 #1095. | |
[2] Edelmann A, Helmuth J, Schwarzer R (2018) Depletion of Human DNA and Reduction of Bacterial Contamination towards a Standardised Method for Infection Diagnosis with Next Generation Sequencing. Poster ECCMID 2018 #P0082. (link) |