Thank You for Visiting US at ECCMID 2018 |
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| 15 May 2018 | |
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We were delighted to present our latest developments for unique automated culture-independent pathogen DNA extraction and broad-range molecular diagnosis of bacteria and fungi to this international audience. We want to thank everyone who visited us at our booth #159 and hope that you enjoyed the meeting as much as we did! |
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In the oral session “Innovations in molecular pathogen detection”, Dr. Rubalskii [1] from Hannover Medical School (Germany) presented his results on a recent study on infectious endocarditis (IE). Automated pathogen enrichment and DNA extraction on the SelectNA™plus robot was used in combination with broad-range 16S/18S rDNA PCR and sequencing analysis (Micro-Dx™) to diagnose 97 samples from 52 patients under suspect of IE. Specimens included mainly excised heart valves and blood, but also pacemaker electrodes, haematomas, pericardial abscesses and thrombi. The SelectNA™plus/Micro-Dx™ analysis resulted in 58% positivity as compared to 42% by culture. The good clinical performance makes SelectNA™plus/Micro-Dx™ a promising tool for culture-independent molecular diagnosis of IE infections as concluded by the speaker. |
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| References | |
| [1] Rubalskii E, Ruemke S, Salmoukas C, Baussmerth C, Keim S, Disqué C, Haverich A, Kühn C (2018) Broad-Range 16S/18S PCR and Sequencing Diagnosis of Infectious Endocarditis Using Automated Pathogen Enrichment and DNA Extraction. Oral presentation ECCMID 2018 #1095. | |
| [2] Edelmann A, Helmuth J, Schwarzer R (2018) Depletion of Human DNA and Reduction of Bacterial Contamination towards a Standardised Method for Infection Diagnosis with Next Generation Sequencing. Poster ECCMID 2018 #P0082. (link) |
With almost 13,000 delegates from 128 countries ECCMID 2018 has been one of the largest meetings in its field, providing the great opportunity to meet world’s leading experts and discuss the latest developments in infectious diseases, infection control and clinical microbiology.
Here are two of our highlights of this year's ECCMID:
Dr. Anke Edelmann and her team [2] from Labor Berlin - Charité Vivantes (Germany) presented a poster on 16S metagenomics with the title “Depletion of human DNA and reduction of bacterial contamination towards a standardised method for infection diagnosis with next generation sequencing”. In this study the impact of different DNA extraction kits regarding depletion of human DNA were compared. All tested DNA extraction kits were further analysed with two master mixes for reagent-borne bacterial DNA contaminations. The authors stated that DNA extraction and master mix reagents have a huge impact on the contamination signature in sequencing reads. Molzym’s human DNA depletion kit and 16S master mix were regarded promising standards to increase bacterial reads and reduce DNA contamination.