NGSeq 16S V3/V4

 Amplicon PCR for 16S library preparation - For Illumina® MiSeq system 

The NGSeq 16S V3/V4 is an ultra-clean master mix for the amplification of eubacterial DNA with universal primers targeting the V3/V4 region of the 16S rRNA gene. The primers include the adaptor sequences suitable for the library preparation for 16S metagenomic sequencing with the Illumina® MiSeq system. The sequences are based on “16S Metagenomic Sequencing Library Preparation” [1] and combinable with the indices for sequencing on the Illumina® MiSeq system.

Fig.1: Schematic drawing of 16S Amplicon PCR using NGSeq 16S V3/V4

The NGSeq 16S V3/V4 contains everything necessary to quickly perform the Amplicon PCR and thus the first stage of the 16S library preparation [1]. The microbial DNA-free reagents offer the possibility to run up to 40 cycles for a higher and more sensitive amplification. Sequencing of contaminations from reagents is prevented allowing a representative view into the bacterial community. Optional real-time PCR can be used to check samples prior to sequencing.

To get a better impression of the benefit, please read either the following blog post or this poster presented at the ECCMID 2018 in Madrid.  

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Reference:

[1] 16S Metagenomic Sequencing Library Preparation; Part # 15044223 Rev.B; https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf