Trends in Molecular Diagnostics               Vol. 5/2019
Hot Topic: Automated Host DNA Depletion and Microbial DNA Isolation
Join us at EMMD Meeting in Noordwijk aan Zee The biennial European Meeting on Molecular Diagnostics (EMMD) will take place the 11^th time in Noordwijk aan Zee, the Netherlands, on 09 - 11 October 2019. Visit our booth #20 and learn more about our innovative solutions for culture-independent molecular diagnosis of pathogens. If you want to schedule your appointment in advance, please e-mail us at info@molzym.com. The Molzym experts are looking forward to meet you in Noordwijk!
New Publications: 1. Impact of pre-operative antimicrobial treatment on microbiological findings from endocardial specimens in infective endocarditis (Halavaara et al. 2019) 2. Technical Information SelectNA™plus – Automated Host DNA Depletion and Microbial DNA Extraction Product Highlights: · Micro-Dx™ CE IVD Sensitive culture-independent diagnosis of body fluids, swabs and tissues by innovative automated pathogen DNA extraction and broad-range 16S/18S rRNA gene PCR and sequencing. · MolYsis-SelectNA™plus Automated microbial DNA enrichment and isolation on the SelectNA™plus robot for reduced hands-on time and increased sensitivity in molecular analysis.
New Publications
1. Impact of pre-operative antimicrobial treatment on microbiological findings from endocardial specimens in infective endocarditis (Halavaara et al. 2019) In their study, Halavaara and colleagues compared the findings of valve culture and 16S rRNA PCR from patients with suspected infective endocarditis regarding the pre-operative antibiotic treatment and the impact of its duration. DNA isolation was done with EasyMag (BioMérieux) at the early stage of the study and was then switched to the SelectNA™plus (Molzym) followed by 16S rRNA PCR. For patients with less than two weeks antimicrobial treatment, 91% of PCR runs were positive and 41% of the cultures. On contrary, for patients with more than two weeks antimicrobial treatment, all valve cultures were negative and PCR was positive in 53%. From negative blood cultures, PCR could detect the pathogen in 77%. In conclusion, PCR aided diagnostics particularly when culture remained negative. >>Request more information >>Reference
2. Technical Information SelectNA™plus – Automated Host DNA Depletion and Microbial DNA Extraction Molzym’s MolYsis™ technology is successfully used in research and clinical diagnostics for many years now. The technology for human DNA depletion prior to microbial DNA isolation is especially valuable for samples with a low load of microorganisms. To reduce the hands-on time and simplify the process for routine laboratories, Molzym automated the whole process, providing the best conditions for molecular analysis without limitations through contaminations. This technical information gives a deeper insight on the characteristics, technology and applications of the SelectNA™plus robot. >>Request more information >>Download Technical Information
Product Highlights
SelectNA™plus robot	Micro-Dx™ CE IVD
Key features: · Bench-top instrument · Flexible: 1 to 12 samples at a time · Vacuum-driven system · UV decontamination For more information, please see above: Technical Information SelectNA™plus.	Fast and precise culture-independent diagnosis of body fluids, swabs and tissues by innovative automated pathogen DNA extraction and broad-range 16S/18S rRNA gene PCR and sequencing analyses. Key features: · Robotic host DNA depletion and isolation of bacterial and fungal DNA · Validated for a diversity of clinical specimens as body fluids, swabs and tissues · Controlled DNA-free reagents and consumables · Identification of pathogens based on 16S and 18S rRNA PCR assays and Sanger sequencing · Extraction and PCR run controls included >>Request more information >>Read more
	MolYsis-SelectNA™plus Automated enrichment and isolation of microbial DNA with minimized contamination risk from air-borne sources and handling errors for increased sensitivity in any molecular assays. Key features: · Automated human and animal DNA depletion and isolation of bacterial and fungal DNA · For body fluids, swabs and tissues · Controlled DNA-free reagents and consumables · Customized analysis >>Request more information >>Read more
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Dear reader, Welcome to this edition of Trends in Molecular Diagnostics. With this newsletter we want to keep you updated on the latest developments in molecular detection of microbial infections. Best regards, Michael Lorenz, Ph. D. (Editor in Chief)
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