Stay informed on topics covering pathogen enrichment and DNA isolation from clinical samples, automation, molecular diagnostics and next generation sequencing. We keep you informed on new developments, give practical tips and provide an inside view on Molzym.
|Pathogen DNA Isolation||NGS||Automation|
|DNA-Free Reagents||Molecular Diagnostics||Newsletter|
Timely diagnosis and treatment are important for successful patient recovery from infections. But in routine microbiology, some results are available with a significant time delay only. Real-time nanopore sequencing was used for rapid pathogen detection in two cases, one of tuberculosis and the other of brucellosis.
Patients with infective endocarditis should immediately receive antibiotic treatment. However, the treatment can hamper the identification of the etiological agents by valve culture. The impact of pre-operatively administered antibiotics was evaluated in a study over six years comparing results of valve culture and direct 16S rRNA gene PCR analysis.
Depletion of host DNA from low microbial load samples is recognized as an effective means for increased target reads and thus deeper analysis of microbiomes. Only a single protocol for diverse liquid and tissue specimens provides standardization and reduction of workload. Get a closer look onto the Ultra-Deep Microbiome Prep kit and its diverse applications.
PCR and sequencing analysis of pathogens without cultivation is an integral part of routine diagnosis. It rapidly uncovers microorganisms that do not grow in culture because of inhibition by antibiotics or fastidious growth requirements. Thereby, PCR/sequencing significantly contributes to the management of patients with bone and joint infections.
Molecular analysis of low microbial load samples is challenging because of the risk of contamination from ubiquitous bacterial DNA. In a recent publication, a workflow for molecular analysis was applied to fingerprint community changes in carious dentine samples before and after dental therapy.