Stay informed on topics covering pathogen enrichment and DNA isolation from clinical samples, automation, molecular diagnostics and Next Generation Sequencing. We keep you informed on new developments, give practical tips and provide an inside view on Molzym.
|Pathogen DNA Isolation||NGS||Automation|
|DNA-Free Reagents||Molecular Diagnostics||Newsletter|
Rapid pathogen detection is essential for timely patient treatment. In contrast to culture, molecular methods provide results within hours. Molzym’s highly sensitive approach combines a unique DNA extraction technique with 16S and 18S rRNA gene PCR and amplicon sequencing for the identification of bacterial and fungal infections. See how it works.
Molzym turns 15 years this month. Over the years, Molzym won reputation as a specialist in the fast molecular diagnosis of microbial pathogens without the need of cultivation. Our expertise in unique DNA extraction solutions and the supply of DNA-free amplification reagents supports the standardisation of qPCR, gene clone library and NGS assaying.
In clinical specimens with low microbial load, host DNA at large excess negatively influences amplification of target pathogen DNA. This contribution guides through Molzym’s MolYsis™ technology of host DNA removal in front of microbial DNA extraction systems. The positive effect of host DNA removal on molecular analysis is discussed.
SelectNA™plus is the ‘Selective extraction of microbial Nucleic Acids’ from mixtures including host and microbial cells providing a ‘plus’ benefit by walk-away automated processing of clinical samples. This highly enriched microbial DNA guarantees highest analytical performance in research and routine diagnosis.
In a poster presentation at this year’s ECCMID congress, Edelmann et al.  compared 4 DNA extraction kits and the influence of human DNA removal for use in microbiome analysis. Additionally, two master mixes were tested for bacterial DNA contamination. An efficient workflow towards a standardised use of NGS in infection diagnosis was proposed.
One of the major challenges in molecular microbe detection are false-positive results due to DNA contaminations of amplification reagents. Molzym’s new proprietary Hot MolTaq 16S/18S is a high quality, DNA-free Taq DNA polymerase ideally suited for the sensitive PCR analysis of microbial target DNA even at very low concentrations. Find out the benefits.