Standard Taq DNA Polymerases

Molzym’s standard high quality products for daily routine PCRs comprise a variety of highly active Taq DNA polymerases. The products suit all applications of PCR and Real-Time PCR assays.

Characters and benefits: Highly active Taq DNA polymerases PCR and Real-Time PCR applications PCR enhancer for GC-rich and difficult templates

MolTaq is Molzym’s highly processive thermostable Taq DNA polymerase with 5’-3’ exonuclease activity useful for research and routine PCR. MolTaq kits can be applied in amplification-based analyses, including exact analyses of SNPs, human, animal and plant genetic markers, molecular epidemiology, molecular ecology as well as animal and plant breeding, food and water quality control, sequencing reactions and gene expression studies.

A variety of kits are available to meet your needs for high performance PCR amplification in daily routine. MolTaq is the standard Taq DNA polymerase kit supplying PCR buffer (1.5mM Mg²⁺) and PCR enhancer for high GC content targets.

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Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15.  

Selected references:

Solbach P, Chhatwal P, Woltemate S, Tacconelli E, Buhl M, Gerhard M, Thoeringer CK, Vehreschild MJGT, Jazmati N, Rupp J, Manns MP, Bachmann O, Suerbaum S (2018) BaiCD gene cluster abundance is negatively correlated with Clostridium difficile infection. PLoS ONE 13(5): e0196977.

Wurdack M, Polidori C, Keller A, Feldhaar H, Schmitt T (2017) Release from prey preservation behavior via prey switch allowed diversification of cuticular hydrocarbon profiles in digger wasps. Evolution 71, 2562-2571.

Shaw LP, Harjunmaa U, Doyle RM, Mulewa S, Charlie D, Maleta K, Callard RE, Walker AS, Balloux F, Ashorn P, Klein N (2016) Distinguishing the signals of gingivitis and periodontitis in supragingival plaque: a cross-sectional cohort study in Malawi. Appl Environ Microbiol 82, 6057–6067. 

Automated Microbial DNA Enrichment and Extraction

Molzym provides a robotic platform and kits for the isolation of microbial DNA from experimental systems and clinical biopsies for molecular analysis. Hands-on time is greatly reduced and reagent-borne DNA contaminations excluded. 

Fully Automated Microbial DNA Extraction:

The SelectNA™plus robotic system is the walk-away solution for enrichment and isolation of microbial DNA. The contained environment in the instrument is ideal to minimize the contamination risk from air-borne sources and handling errors. 

The robot is operated with the MolYsis-SelectNA™plus kit and validated for 1 ml fluid samples, swabs and tissue biopsies. The process is based on an innovative vacuum-driven protocol for host DNA depletion and pathogen enrichment followed by microbial DNA isolation providing an optimal solution for e.g. low load samples with only small amounts of microbial DNA. The isolated DNA can be used with any molecular assay for the analysis of bacterial or fungal genes, genomes or metagenomes.

Characters and benefits: Innovative walk-away pathogen DNA extraction Host-DNA-depletion technology DNA-free consumables and reagents Vacuum-driven system UV decontamination Validated for primary sterile body fluids, swabs and tissues Bench-top instrument Flexible: 1 to 12 samples at a time

Order information:

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Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein!, call at +49 (0) 421 / 69 61 62 17 or visit our Blog.  

DNA-Free PCR Reagents & Assays

PCR and Real-Time PCR Reagents:

Characters and benefits: High activity, DNA-free Taq DNA polymerases Hot start Taq available PCR amplification up to 40 cycles without background Master mixes for PCR and Real-Time PCR (fluorescent dye) Taq and master mixes are ready for probe assays

A series of kits is especially dedicated to the sensitive and specific detection of bacterial and fungal DNA in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance, allowing up to 40 amplification cycles without background in negative controls. DNA-free MolTaq 16S/18S and Hot MolTaq 16S/18S are the Taq DNA polymerases of choice if you experience contamination problems with other standard Taqs. The new developed Hot MolTaq 16S/18S combines the favourable characters of MolTaq 16S/18S with hot start amplification and thereby increased specificity. 

Master mixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, and Mg²⁺ (3mM final concentration). Mastermix 16S/18S Basic is a ready-to-go master mix – just add your validated PCR primers and probes. Mastermix 16S/18S Dye is the master mix of choice if you want to run Real-Time PCRs with your primers using an intercalating dye.

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Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.  

Broad-Range 16S & 18S Assays:

Assays are available for the detection of bacterial and fungal DNA. They possess high activity for the amplification of hypervariable regions of the 16S and 18S rRNA genes down to femtogram amounts.

Characters and benefits High activity, DNA-free Bacterial 16S target: V3/V4 hypervariable region Fungal 18S target: V8/V9 hypervariable region Up to 40 cycles without background PCR and Real-Time PCR (fluorescent dye) assays Sequencing primers available for Sanger sequencing

Many applications demand a very sensitive and rapid detection of bacterial and/or fungal DNA. Such applications comprise microbial DNA analysis in clinics, quality testing of food and pharma products and contamination control of biotechnological processes. Molzym offers PCR and Real-Time PCR assays that test for even traces of bacterial and fungal DNA in a sample. Manufacturing under strict quality measures for the absence of DNA contamination guarantees reliable results from test to test.

Order information:

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Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.  

Selected references:

Tardif KD, Schlaberg R (2017) Development of a real-time PCR assay for the direct detection of Candida species causing vulvovaginal candidiasis. Diagn Microbiol Inf Dis, doi.org/10.1016/j.diagmicrobio.2017.01.012.

Hammer A, Wolff D, Geißdörfer W, Schrey M, Ziegler R, Steiner HH, Bogdan C (2017) A spinal epidural abscess due to Streptobacillus moniliformis infection following a rat bite: case report. J Neurosurg, DOI: 10.3171/2016.12.SPINE161042.

Moore MS, McCarroll MG, McCann CD, May L, Younes N, Jordan JA (2016) Direct screening of blood by PCR and pyrosequencing for a 16S rRNA gene target from emergency department and intensive care unit patients being evaluated for bloodstream infection. J Clin Microbiol 54, 99–105.

Kacerovsky M, Musilova I, Jacobsson B, Drahosova M, Hornychova H, Rezac A, Andrys C (2015) Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes. J Maternal-Fetal Neonatal 28, 1116-1122.

Ultra-Deep Microbiome Prep

The Ultra-Deep Microbiome Prep is the perfect kit for ultra-sensitive identification of a broad range of bacteria and fungi from a variety of sample types. DNA extraction is supplied for whole blood, primary body fluids, including CSF and synovial fluid, BAL, exudates like sputum and urine, human and animal tissues, biopsies, and biofilms. As an important character the Ultra-Deep Microbiome Prep enables detecting and identifying microorganisms that are intact but not growing in culture.

Characteristics and benefits: Isolation of enriched microbiome DNA (bacteria & fungi) Depletion of irrelevant human/animal and “free” microbial DNA DNA-free reagents and plastics enabling contamination-free, ultra-deep microbiome bioanalyses Small and large volumes: 0.2 to 10 ml liquid samples and tissues

The Ultra-Deep Microbiome Prep Kit is the ultimate solution combining protocols for the depletion of human/animal host DNA and extraction of enriched microbial DNA. The Ultra-Deep Microbiome Prep allows for significantly deeper sequencing compared to conventional DNA isolation and all reagents, plastics and spin columns provided are free of contaminations.

Order information:

Download section:

Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.  

Selected references:

Chalupova M, Skalova A, Hajek T, Geigerova L, Kralova D, Liska P, Hecova H, Molacek J, Hrabak J (2018) Bacterial DNA detected on pathologically changed heart valves using 16S rRNA gene amplification. Folia Microbiol (2018) 63: 707. https://doi.org/10.1007/s12223-018-0611-6

Costantini L, Magno S, Albanese D, Donati C, Molinari R, Filippone A, Masetti R, Merendino N (2018) Characterization of human breast tissue microbiota from core needle biopsies through the analysis of multi hypervariable 16S-rRNA gene regions. Scientific Reports (2018) 8:16893. https://doi.org/10.1038/s41598-018-35329-z

Leo S, Gaïa N, Ruppé E, Emonet S, Girard M, Lazarevic V, Schrenzel J (2017) Detection of Bacterial Pathogens from Broncho-Alveolar Lavage by Next-Generation Sequencing. International Journal of Molecular Sciences. 2017;18(9):2011. https://doi.org/10.3390/ijms18092011