Clinical Diagnostics

Automated Pathogen Diagnosis 

Fully Automated Pathogen Diagnosis Semi-Automated Pathogen Diagnosis

Micro-Dx™ CE IVD

Innovative automated pathogen DNA enrichment and isolation on SelectNA™plus robot combined with broad-range 16S & 18S PCR assays 

UMD-SelectNA™ CE IVD

Combination of manual pathogen enrichment and automated DNA isolation and broad-range 16S & 18S PCR assays

 

Micro-Dx™ CE IVD (not available in USA)

Good bye enervating DNA preparation – CE IVD-marked Micro-Dx™ presents automated DNA preparation from diverse specimens. Low hands-on in Real-Time PCR diagnosis of pathogens is ideal for daily routine. 
 prod SelectNAplus 09 frei 3 4

Characters and benefits:

  • Completely automated human DNA removal and microbial DNA extraction
  • Robotic isolation of bacterial and fungal DNA
  • Low hands-on time (10-15 min)
  • Just one protocol for a diversity of clinical specimens
  • Culture-independent diagnosis of growing and non-growing pathogens
  • Sequencing identification of pathogens from 160 bacterial genera
  • Exact discrimination of Candida, Cryptococcus and other yeast species, Aspergillus
  • Flexibility: processing of 1-12 samples per run
  • Controlled DNA-free reagents and consumables
  • Extraction and PCR run controls included

Micro-Dx™ is the culture-independent molecular analysis of bacterial and fungal pathogens in clinical specimens with fully automated extraction of microbial DNA by the SelectNA™plus robot. The test is validated for a variety of clinical specimens, including whole blood, CSF, BAL, joint aspirates, ascites fluid, peritoneal fluid, pus, wound and nasal swabs, bone and soft tissue biopsies, heart and aortic valves and more.

Micro-Dx™ does the extraction of microbial DNA in a completely automated, walk-away manner. The eluted DNA is depleted of human DNA which promotes PCR or Real-Time PCR analysis by the supplied DNA-free reagents to high detection levels and hence diagnostic accuracy.

The robotic extraction of DNA from a variety of specimens and the unbiased, broad-range identification of bacteria and fungi make Micro-Dx™ a valuable tool in the daily in-vitro diagnosis of pathogens. 

 

Order information:

Kit
Application
Content
Order No.
Quotation Request

Micro-DxCE IVD

Fluids, tissue biopsies, swabs
24 reactions
U-200-024
48 reactions U-200-048
SelectNA™plus robot Instrument for fully automated microbial DNA extraction
1
D-400-001

Download product flyer 

Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein!, call at +49 (0) 421 / 69 61 62 17 or visit our Blog.  

 

Download open list of microorganisms found in clinical and other specimens by sequencing (2018)

 

Selected references:

Lustig M (2018) Clinical evaluation of Micro-Dx™ CE IVD – eubacterial and panfungal PCR diagnosis using automated DNA extraction. Trends in Molecular Diagnostics, no. 1/18: 1-2 (Molzym application note).

Rubalskii E, Ruemke S, Salmoukas C, Baussmerth C, Keim S, Linow M, Disqué C, Haverich A, Kühn C (2018) Broad-range 16S/18S PCR and sequencing diagnosis of infectious endocarditis using automated pathogen enrichment and DNA extraction. Poster, Infectious Diseases Diagnostics Conference, London, UK (Poster)

Schubert S (2017) Automated microbial DNA isolation from clinical samples for routine broad-range real‐time PCR analysis and sequence identification. Oral presentation no. OS0779, ECCMID, Wien, Austria (Presentation)

 

 

UMD-SelectNA™ CE IVD (not available in USA): 

UMD-SelectNA™ is a kit that uses semi-automated pathogen DNA isolation from clinical samples, broad-range 16S and 18S rRNA gene PCR or Real-Time PCR and amplicon Sanger sequencing for pathogen diagnosis.
 prod UMD SelectNA 300

UMD-SelectNA™ enables human DNA removal in a manual procedure followed by magnetic beads-driven microbial DNA extraction on the following instruments:

  • Liaison® Ixt (Diasorin),
  • Arrow® (Nordiag),
  • Seeprep12™ (Seegene) or
  • GenoXtract® (Hain Lifescience)
 

Order information:

Kit
Application
Content
Order No.
Quotation Request

UMD-SelectNACE IVD

Fluids, tissue biopsies, swabs
24 reactions
U-050-024
48 reactions U-050-048

Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein!, call at +49 (0) 421 / 69 61 62 17 or visit our Blog

 

Download open list of microorganisms found in clinical and other specimens by sequencing (2018)

 

Selected references:

Johannesen, KM, Kolekar, SB, Greve, N et al. (2019) Differences in mortality in Fusobacterium necrophorum and Fusobacterium nucleatum infections detected by culture and 16S rRNA gene sequencing. Eur J Clin Microbiol Infect Dis (2019) 38: 75. https://doi.org/10.1007/s10096-018-3394-4 

Tkadlec J, Peckova M, Sramkova L, Rohn V, Jahoda D, Raszka D, Berousek J, Mosna F, Vymazal T, Kvapil M, Drevinek P (2018) The use of broad-range bacterial PCR in the diagnosis of infectious diseases: a prospective cohort study. Clin Microbiol Infect. 2018 Oct 12. pii: S1198-743X(18)30668-2. DOI: 10.1016/j.cmi.2018.10.001

Stavnsbjerg C, Niels Frimodt-Møller N, Claus Moser C, Thomas Bjarnsholt T (2017) Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples. BMC Infectious Diseases, 2017, Volume 17, Number 1, Page 1, https://doi.org/10.1186/s12879-017-2333-9