Sequence Analysis Software - SepsiTest™-BLAST

A free online tool is available for the BLAST analysis of sequences of amplicons generated from positive Molzym tests and assays. The library contains >7,000 high quality sequences of 16S and 18S rRNA genes.

Characters and benefits: More than 7,000 quality-checked sequences Complete 16S and 18S rRNA gene sequences of bacteria and fungi Easy sequence input by copy-paste or FASTA-file upload Clear output of strain designation

Sequencing strategy

Molzym’s diagnostic tests and assays taking amplicon sequences as the ultimate approach for species discrimination. Three sequencing primers are provided: SeqGP16, SeqGN16 and SeqYeast18. Amplicons from Assay MA Yeast / Mastermix 18S Complete are sequenced with SeqYeast18. For sequencing of amplicons from Assay MA Bac / Mastermix 16S Complete, SeqGP16 and SeqGN16 are used in separate reactions, binding to regions specific for Gram-positive and Gram-negative bacteria. Although SeqGN16 targets mainly Gram-negative bacteria and SeqGP16 mainly Gram-positive bacteria, both sequencing primers present exceptions. A few Gram-negative species will be detected by the SeqGP16 and some Gram-positive species will be detected by the SeqGN16. The exceptions of the sequencing primers are listed here: Exceptions of Sequencing Primers.

SepsiTest™-BLAST

Molzym provides a free online tool, SepsiTest™-BLAST that aligns sequences of amplicons to quality-edited sequences and reads out the closest relationship to a species in the library. By using the tool, results are provided within seconds.

Go to SepsiTest™-BLAST

For a fast overview and your search for particular organisms, we offer an alphabetical sorted list of all SepsiTest™-BLAST entries within an Excel file - download file. 

Contact:

Project manager: Dr. Michael Lustig
Tel.: +49 (0) 421 / 69 61 62 0
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