Life Science

Pathogen DNA Enrichment and Isolation

MolYsis™ kits are ideal for the supply of enriched microbial DNA for the direct analysis of pathogens in human and animal samples. The kits are evaluated for all sorts of body fluids like blood, CSF, synovial aspirates, BAL and other specimens.

prod teaser molysis

Characters and benefits:

  • Depletion of host (human/animal) DNA from body fluids
  • Enrichment of bacterial and fungal DNA
  • Removal of PCR inhibitors
  • Broad-range lysis of Gram-positive, Gram-negative bacteria and fungi
  • Buffers and reagents are contamination-free
  • 0.2 to 10 ml sample volumes
  
MolYsis™ is a unique system of targeted isolation of microbial DNA from complex clinical samples such as blood, aspirates and other body fluids. The selective lysis of host cells and the degradation of the released host DNA avoid unspecific primer binding and thereby greatly enhance the sensitivity and specificity of PCR or Real-Time PCR analysis of pathogens. For detailed information see Technology. The kit series allows sample processing in the range 0.2 to 10 ml.
 
Molzym offers pre-treatment MolYsis™ Basic kits for host DNA depletion that can be combined with any microbial DNA isolation kit established in the laboratory, including manual and automated systems.  
The MolYsis™ Complete kit series provides protocols for the whole process, ranging from pre-treatment for host DNA depletion to microbial DNA isolation.
 
All kit ingredients, including buffers, reagents and consumables, are quality-checked for the absence of microbial DNA contaminations. This secures precise molecular analysis.
 
The MolYsis™ kits are ideally combinable with Molzym’s DNA-free PCR/Real-Time PCR reagents and detection assays, MolTaq 16S/18S, Hot-Moltaq 16S/18S and Mastermix 16S/18S kits.
 

Order information:

Kits
Sample
Sample Volume
Order No.

Quotation Request 

Host DNA depletion    
MolYsis™ Basic
liquid biopsy
0.2 ml
D-300-050, D-300-100 Request form
MolYsis™ Basic5
liquid biopsy
0.2-1 ml or 5 ml
D-301-050, D-301-100
MolYsis™ Basic10 liquid biopsy 5-10 ml D-305-050, D-305-100  
Host DNA depletion and microbial DNA isolation    
MolYsis™ Complete5
liquid biopsy
0.2-1 ml or 5 ml
D-321-050, D-321-100
MolYsis™ Complete10 liquid biopsy 5-10 ml D-325-050, D-325-100 

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Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.  

 

Download open list of microorganisms found in clinical and other specimens by sequencing (2018)

 

Selected references:

Krohn S , Zeller K, Böhm S, Chatzinotas A, Harms H, Hartmann J, Heidtmann A, Herber A, Kaiser T, Treuheit M, Hoffmeister A, Berg T, Engelmann C (2018) Molecular quantification and differentiation of Candida species in biological pecimens of patients with liver cirrhosis. PLOS One 13(6), e0197319. https://www.ncbi.nlm.nih.gov/pubmed/29897895

Schmidt K, Mwaigwisya S, Crossman LC, Doumith M, Munroe D, Pires C, Khan AM, Woodford N, Saunders NJ, Wain J, O’Grady J, Livermore DM (2017) Identification of bacterial pathogens and antimicrobial resistance directly from clinical urines by nanopore-based metagenomic sequencing. J Antimicrob Chemother 2017; 72: 104–114. https://doi.org/10.1093/jac/dkw397

Thoendel M, Jeraldo PR, Greenwood-Quaintance KE, Yao JZ, Chia N, Hanssen AD, Abdel MP, Patel R (2016) Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing. Journal of Microbiological Methods 127 (2016) 141–145. http://dx.doi.org/10.1016/j.mimet.2016.05.022