16S Pathogen Diagnosis of Culture-Negative Infections |
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22 January 2019 | |
The UMD-SelectNA™ found bacterial DNA in 22 samples (29% positive), whereas only 11 samples were positive with MicroSeq® ID (14%). Ten samples from 8 patients with endocarditis, abscesses, infected joint and cancer were positive with both methods. Pathogens were identical by the two assays, except for a mixed infection in a pus sample from a cerebral abscess where the compositions of species were different but regarded relevant with both methods. UMD-SelectNA™ was further positive with 12 samples that were MicroSeq® ID-negative. Among these, other findings from the patients supported the results of 7 positive samples, whereas 5 samples gave ambiguous results. The authors concluded that UMD-SelectNA™ found significantly more pathogens in culture-negative samples and thus proved more sensitive than MicroSeq® ID. Because of the increased sensitivity special care has to be taken to avoid contamination during handling. Clinical evaluation of the patients' history needs to be performed to assess the relevance of findings. |
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Reference | |
[1] Stavnsbjerg C, Frimodt-Møller N, Moser C, Bjarnsholt T (2017) Comparison of two commercial broad range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples. BMC Inf Dis 17, 233 DOI 10.1186/s12879-017-2333-9. | |
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