16S rDNA PCR and Sequencing Diagnosis of Bone and Joint Infections
|09 September 2019|
Among three studies, including 482 patients in total, the diagnostic sensitivity of the Molzym tests, SepsiTest™-UMD and semi-automated UMD-SelectNA™, respectively, against culture was 89 to 100%. A high proportion (13 to 29%) of samples were PCR-positive, but culture-negative. One study judged the PCR-positive and sequencing results (26%) of added value in 20% of samples. Usual pathogens of orthopaedic infections, including staphylococci and streptococci, as well as rare pathogens were identified, including Granulicatella adiacens, Finegoldia magna, Tropheryma whipplei and Parvimonas micra.
In summary, the molecular tests were regarded beneficial by their contribution to patient management in cases where cultures are negative.
| Lustig M (2019) Application note: Culture-independent broad-range 16S rDNA PCR and sequencing diagnosis of bone and joint infections. Trends in Molecular Diagnostics No. 2/2019, 1-2. (link)|