DNA-Free PCR Reagents & Assays

PCR and Real-Time PCR Reagents:

Characters and benefits: High activity, DNA-free Taq DNA polymerases Hot start Taq available PCR amplification up to 40 cycles without background Master mixes for PCR and Real-Time PCR (fluorescent dye) Taq and master mixes are ready for probe assays

A series of kits is especially dedicated to the sensitive and specific detection of bacterial and fungal DNA in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance, allowing up to 40 amplification cycles without background in negative controls. DNA-free MolTaq 16S/18S and Hot MolTaq 16S/18S are the Taq DNA polymerases of choice if you experience contamination problems with other standard Taqs. The new developed Hot MolTaq 16S/18S combines the favourable characters of MolTaq 16S/18S with hot start amplification and thereby increased specificity. 

Master mixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, and Mg²⁺ (3mM final concentration). Mastermix 16S/18S Basic is a ready-to-go master mix – just add your validated PCR primers and probes. Mastermix 16S/18S Dye is the master mix of choice if you want to run Real-Time PCRs with your primers using an intercalating dye.

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Broad-Range 16S & 18S Assays:

Assays are available for the detection of bacterial and fungal DNA. They possess high activity for the amplification of hypervariable regions of the 16S and 18S rRNA genes down to femtogram amounts.

Characters and benefits High activity, DNA-free Bacterial 16S target: V3/V4 hypervariable region Fungal 18S target: V8/V9 hypervariable region Up to 40 cycles without background PCR and Real-Time PCR (fluorescent dye) assays Sequencing primers available for Sanger sequencing

Many applications demand a very sensitive and rapid detection of bacterial and/or fungal DNA. Such applications comprise microbial DNA analysis in clinics, quality testing of food and pharma products and contamination control of biotechnological processes. Molzym offers PCR and Real-Time PCR assays that test for even traces of bacterial and fungal DNA in a sample. Manufacturing under strict quality measures for the absence of DNA contamination guarantees reliable results from test to test.

Order information:

Download section:

Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.  

Selected references:

Tardif KD, Schlaberg R (2017) Development of a real-time PCR assay for the direct detection of Candida species causing vulvovaginal candidiasis. Diagn Microbiol Inf Dis, doi.org/10.1016/j.diagmicrobio.2017.01.012.

Hammer A, Wolff D, Geißdörfer W, Schrey M, Ziegler R, Steiner HH, Bogdan C (2017) A spinal epidural abscess due to Streptobacillus moniliformis infection following a rat bite: case report. J Neurosurg, DOI: 10.3171/2016.12.SPINE161042.

Moore MS, McCarroll MG, McCann CD, May L, Younes N, Jordan JA (2016) Direct screening of blood by PCR and pyrosequencing for a 16S rRNA gene target from emergency department and intensive care unit patients being evaluated for bloodstream infection. J Clin Microbiol 54, 99–105.

Kacerovsky M, Musilova I, Jacobsson B, Drahosova M, Hornychova H, Rezac A, Andrys C (2015) Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes. J Maternal-Fetal Neonatal 28, 1116-1122.