DNA-Free PCR Reagents & Assays
|PCR and Real-Time PCR Reagents
|Broad-Range 16S & 18S Assays
|Polymerases, water and master mixes are manufactured free of microbial DNA and are high in amplification activity over 40 cycles. Master mixes contain all reagents for assays to be used with custom primers.
|Complete assays are available for the sensitive PCR/Real-Time PCR detection of parts of 16S and 18S rRNA genes of eubacteria and fungi, respectively. Amplicon sequencing primers enable their identification.
Characters and benefits:
A series of kits is especially dedicated to the sensitive and specific detection of bacterial and fungal DNA in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance, allowing up to 40 amplification cycles without background in negative controls. DNA-free MolTaq 16S/18S and Hot MolTaq 16S/18S are the Taq DNA polymerases of choice if you experience contamination problems with other standard Taqs. The new developed Hot MolTaq 16S/18S combines the favourable characters of MolTaq 16S/18S with hot start amplification and thereby increased specificity.
Master mixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, and Mg2+ (3mM final concentration). Mastermix 16S/18S Basic is a ready-to-go master mix – just add your validated PCR primers and probes. Mastermix 16S/18S Dye is the master mix of choice if you want to run Real-Time PCRs with your primers using an intercalating dye.
|DNA-free Taq DNA Polymerases
|Amplification of bacterial and fungal sequences
|Hot MolTaq 16S/18S
|Hot start amplification of bacterial and fungal sequences
|DNA-free Master Mixes
|Mastermix 16S/18S Basic
|Master mix for use with custom primers and probes
|S-040-0100, S-040-0250, S-040-1000
|Mastermix 16S/18S Dye
|Master mix for custom Real-Time PCR assays
|S-030-0100, S-030-0250, S-030-1000
|DNA-free Water, PCR-grade
|For negative control PCR runs
Assays are available for the detection of bacterial and fungal DNA. They possess high activity for the amplification of hypervariable regions of the 16S and 18S rRNA genes down to femtogram amounts.
Characters and benefits
Many applications demand a very sensitive and rapid detection of bacterial and/or fungal DNA. Such applications comprise microbial DNA analysis in clinics, quality testing of food and pharma products and contamination control of biotechnological processes. Molzym offers PCR and Real-Time PCR assays that test for even traces of bacterial and fungal DNA in a sample. Manufacturing under strict quality measures for the absence of DNA contamination guarantees reliable results from test to test.
Ultra-Clean Bacterial DNA Assays
Mastermix 16S Complete
Ready-to-use Real-Time PCR assay targeting the 16S V3/V4 region
S-020-0100, S-020-0250, S-020-1000
Ultra-Clean Fungal DNA Assay
Mastermix 18S Complete
Ready-to-use Real-Time PCR assay targeting the 18S V8/V9 region
S-070-0100, S-070-0250, S-070-1000
Set of Eubacterial Sequencing Primers
Set of sequencing primers, eubacterial (16S V3/V4)
Panfungal Sequencing Primer
Sequencing primer, fungal (18S V8/V9)
|DNA-free PCR Reagents Product Flyer (English)
|DNA-free Water Product Flyer (English)
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