Life Science

Pathogen DNA Enrichment and Isolation

MolYsis™ kits are ideal for the supply of enriched microbial DNA for the direct analysis of pathogens in human and animal samples. The kits are evaluated for all sorts of body fluids like blood, CSF, synovial aspirates, BAL and other specimens.

prod teaser molysis

Characters and benefits:

  • Depletion of host (human/animal) DNA from body fluids
  • Enrichment of bacterial and fungal DNA
  • Removal of PCR inhibitors
  • Broad-range lysis of Gram-positive, Gram-negative bacteria and fungi
  • Buffers and reagents are contamination-free
  • 0.2 to 1 ml or 5 ml sample volumes
  
MolYsis™ is a unique system of targeted isolation of microbial DNA from complex clinical samples such as blood, aspirates and other body fluids. The selective lysis of host cells and the degradation of the released host DNA avoid unspecific primer binding and thereby greatly enhance the sensitivity and specificity of PCR or Real-Time PCR analysis of pathogens. For detailed information see Technology. The kit series allows sample processing in the range of 0.2 to 1 ml or 5 ml.
 
Molzym offers a pre-treatment MolYsis™ Basic kit for host DNA depletion that can be combined with any microbial DNA isolation kit established in the laboratory, including manual and automated systems.  
The MolYsis™ Complete kit series provides protocols for the whole process, ranging from pre-treatment for host DNA depletion to microbial DNA isolation.
 
The MolYsis™ technology is also applicable for tissue and swab samples. With the Ultra-Deep Microbiome Prep kit ≤1 ml liquid and tissue specimens can be used. Highest flexibility is given with the Ultra-Deep Microbiome Prep10 kit for volumes from 1-10 ml and tissues.
 
All kit ingredients, including buffers, reagents and consumables, are quality-checked for the absence of microbial DNA contaminations. This secures precise molecular analysis.
 
The MolYsis™ kits are ideally combinable with Molzym’s DNA-free PCR/Real-Time PCR reagents and detection assays, MolTaq 16S/18S, Hot-Moltaq 16S/18S and Mastermix 16S/18S kits.
 
 
Automation: The process of microbial DNA enrichment and isolation is also available fully automated. Click here for more information.
 
 

Order information:

Kits
Sample
Sample Volume
 Order No.

Quotation Request 
Host DNA depletion    
MolYsis™ Basic5
liquid biopsy
0.2-1 ml or 5 ml
 D-301-050, D-301-100 Request form
Host DNA depletion and microbial DNA isolation    
MolYsis™ Complete5
liquid biopsy
0.2-1 ml or 5 ml
 D-321-050, D-321-100
Host DNA depletion and microbial DNA isolation from liquid and tissue specimens
Ultra-Deep Microbiome Prep liquid biopsy and tissue ≤1 ml and tissue G-020-025, G-020-050
Ultra-Deep Microbiome Prep10 liquid biopsy and tissue 1-10 ml and tissue G-030-025, G-030-050


Download section: 

 Product Flyer Version
 MolYsis™ Product Flyer (English) V06
 MolYsis™ Product Flyer (German) V01
 Manuals Version
 MolYsis™ Basic5 Manual (English) V09
 MolYsis™ Complete5 Manual (English)  V09
 Ultra-Deep Microbiome Prep Manual (English) V07
 Ultra-Deep Microbiome Prep Short Manual (English) V05
 Ultra-Deep Microbiome Prep10 Manual (English) V06 
 Ultra-Deep Microbiome Prep10 Short Manual (English) V05 
 Other Version
 Open list of microorganisms found in clinical and other specimens by sequencing March 2023
 Flyers Versions Overview August 2024

 

Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.  

 

Selected references:

Krohn S , Zeller K, Böhm S, Chatzinotas A, Harms H, Hartmann J, Heidtmann A, Herber A, Kaiser T, Treuheit M, Hoffmeister A, Berg T, Engelmann C (2018) Molecular quantification and differentiation of Candida species in biological pecimens of patients with liver cirrhosis. PLOS One 13(6), e0197319. https://www.ncbi.nlm.nih.gov/pubmed/29897895

Schmidt K, Mwaigwisya S, Crossman LC, Doumith M, Munroe D, Pires C, Khan AM, Woodford N, Saunders NJ, Wain J, O’Grady J, Livermore DM (2017) Identification of bacterial pathogens and antimicrobial resistance directly from clinical urines by nanopore-based metagenomic sequencing. J Antimicrob Chemother 2017; 72: 104–114. https://doi.org/10.1093/jac/dkw397

Thoendel M, Jeraldo PR, Greenwood-Quaintance KE, Yao JZ, Chia N, Hanssen AD, Abdel MP, Patel R (2016) Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing. Journal of Microbiological Methods 127 (2016) 141–145. http://dx.doi.org/10.1016/j.mimet.2016.05.022