Life Science

Ultra-Deep Microbiome Prep

The Ultra-Deep Microbiome Prep is the perfect kit for ultra-sensitive identification of a broad range of bacteria and fungi from a variety of sample types. DNA extraction is supplied for whole blood, primary body fluids, including CSF and synovial fluid, BAL, exudates like sputum and urine, human and animal tissues, biopsies, and biofilms. As an important character the Ultra-Deep Microbiome Prep enables detecting and identifying microorganisms that are intact but not growing in culture.

prod ngs extraction

Characteristics and benefits:

  • Isolation of enriched microbiome DNA (bacteria & fungi)
  • Depletion of irrelevant human/animal and “free” microbial DNA
  • DNA-free reagents and plastics enabling contamination-free, ultra-deep microbiome bioanalyses
  • Small and large volumes: 0.2 to 10 ml liquid samples and tissues

The Ultra-Deep Microbiome Prep Kit is the ultimate solution combining protocols for the depletion of human/animal host DNA and extraction of enriched microbial DNA. The Ultra-Deep Microbiome Prep allows for significantly deeper sequencing compared to conventional DNA isolation and all reagents, plastics and spin columns provided are free of contaminations.

 
Related products:
 
More kits for host DNA depletion and microbial DNA isolation for liquid samples can be found in the chapter Pathogen DNA Isolation.
 
Automation: The process of microbial DNA enrichment and isolation from body fluids and tissue samples is also available semi- and fully automated. Click here for more information.

 

Order information:

Kit
Application
Content
Order No.
Quotation Request
Ultra-Deep Microbiome Prep
Host DNA depletion and microbial DNA isolation from 0.2-1 ml liquid sample or 0.25cm² tissue sample
25 reactions
G-020-025
50 reactions
G-020-050
Ultra-Deep Microbiome Prep10
Host DNA depletion and microbial DNA isolation from 1-10 ml liquid sample or 0.25cm² tissue sample
25 reactions
G-030-025
50 reactions
G-030-050

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Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.  

 

Download open list of microorganisms found in clinical and other specimens by sequencing

 

Selected references:

Chalupova M, Skalova A, Hajek T, Geigerova L, Kralova D, Liska P, Hecova H, Molacek J, Hrabak J (2018) Bacterial DNA detected on pathologically changed heart valves using 16S rRNA gene amplification. Folia Microbiol (2018) 63: 707. https://doi.org/10.1007/s12223-018-0611-6

Costantini L, Magno S, Albanese D, Donati C, Molinari R, Filippone A, Masetti R, Merendino N (2018) Characterization of human breast tissue microbiota from core needle biopsies through the analysis of multi hypervariable 16S-rRNA gene regions. Scientific Reports (2018) 8:16893. https://doi.org/10.1038/s41598-018-35329-z

Leo S, Gaïa N, Ruppé E, Emonet S, Girard M, Lazarevic V, Schrenzel J (2017) Detection of Bacterial Pathogens from Broncho-Alveolar Lavage by Next-Generation Sequencing. International Journal of Molecular Sciences. 2017;18(9):2011. https://doi.org/10.3390/ijms18092011