Ultra-Deep Microbiome Prep
The Ultra-Deep Microbiome Prep is the perfect kit for ultra-sensitive identification of a broad range of bacteria and fungi from a variety of sample types. DNA extraction is supplied for whole blood, primary body fluids, including CSF and synovial fluid, BAL, exudates like sputum and urine, human and animal tissues, biopsies, and biofilms. As an important character the Ultra-Deep Microbiome Prep enables detecting and identifying microorganisms that are intact but not growing in culture.
Characteristics and benefits:
|
The Ultra-Deep Microbiome Prep Kit is the ultimate solution combining protocols for the depletion of human/animal host DNA and extraction of enriched microbial DNA. The Ultra-Deep Microbiome Prep allows for significantly deeper sequencing compared to conventional DNA isolation and all reagents, plastics and spin columns provided are free of contaminations.
Order information:
Kit
|
Application
|
Content
|
Order No.
|
Quotation Request
|
Ultra-Deep Microbiome Prep
|
Host DNA depletion and microbial DNA isolation from 0.2-1 ml liquid sample or 0.25cm² tissue sample
|
25 reactions
|
G-020-025
|
|
50 reactions
|
G-020-050
|
|||
Ultra-Deep Microbiome Prep10
|
Host DNA depletion and microbial DNA isolation from 1-10 ml liquid sample or 0.25cm² tissue sample
|
25 reactions
|
G-030-025
|
|
50 reactions
|
G-030-050
|
Download section:
Product Flyer | Version |
Ultra-Deep Microbiome Prep Product Flyer (English) | V 1.1.2015 |
Manuals | Version |
Ultra-Deep Microbiome Prep Manual (English) | V07 |
Ultra-Deep Microbiome Prep Short Manual (English) | V05 |
Ultra-Deep Microbiome Prep10 Manual (English) | V06 |
Ultra-Deep Microbiome Prep10 Short Manual (English) | V05 |
Other | Version |
Open list of microorganisms found in clinical and other specimens by sequencing | March 2023 |
Please inquire for more detailed information, applications and references at Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein! or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.
Selected references:
Chalupova M, Skalova A, Hajek T, Geigerova L, Kralova D, Liska P, Hecova H, Molacek J, Hrabak J (2018) Bacterial DNA detected on pathologically changed heart valves using 16S rRNA gene amplification. Folia Microbiol (2018) 63: 707. https://doi.org/10.1007/s12223-018-0611-6
Costantini L, Magno S, Albanese D, Donati C, Molinari R, Filippone A, Masetti R, Merendino N (2018) Characterization of human breast tissue microbiota from core needle biopsies through the analysis of multi hypervariable 16S-rRNA gene regions. Scientific Reports (2018) 8:16893. https://doi.org/10.1038/s41598-018-35329-z
Leo S, Gaïa N, Ruppé E, Emonet S, Girard M, Lazarevic V, Schrenzel J (2017) Detection of Bacterial Pathogens from Broncho-Alveolar Lavage by Next-Generation Sequencing. International Journal of Molecular Sciences. 2017;18(9):2011. https://doi.org/10.3390/ijms18092011